BIOASSAY OF D-TUBOCURARINE PDF

BIOASSAY OF INSULIN BIOASSAY OF D-TUBOCURARINE MERITS OF BIOASSAY DEMERITS OF BIOASSAY REFRENCES. is being done through. Bioassay, official method, adrenaline, digitalis, d-tubocurarine, heparin, insulin, oxytocin, gonadotropin, chorionic gonadotropin, urokinase, streptokinase. Second 4 x 4 latin square bioassay was performed using a series: with a The presence of d-tubocurarine was demonstrated in the Peruvian.

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It is a perfect in vitro model. Mean dose which produces head drop of the test sample is compared with the mean dose of standard preparation. A frog is pithed and laid on its back on a cork covered board to which it is pinned. Acetylcholine contraction is recorded on slow moving drum for 90 sec.

The responses are recorded using isotonic frontal writing lever with 1 G. Location The rectus abdominis is a long flat muscle, which extends along the whole length of the front of the abdomen.

In order to view it, please contact the author of the presentation. Cross-over test is carried out to minimise biological error due to animal variation. Two similar contractions with the same concentration of acetylcholine are obtained.

Animals should be free from disease, obtained from a healthy colony and should be accustomed with the experimental procedure. Bioassay of D- Tubocurarine. College of Pharmacy, Ahmedabad. The percentage reduction at each dose levels is calculated and log dose response curve of the standard drug is plotted. Screening Methods In Pharmacology. Rabbit head drop method for the bioassay of d-tubocurarine.

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The muscle is stabilized for min. The presentation is successfully added In D-tubocurzrine Favorites. Head drop after injection.

Infusion is continued till the rabbit will not be in a position to hold its head erect or there will be no response by focussing light on the eyes and the neck gets elongated and toneless. Helps to flexing lumbar spine.

PHARMACOLOGY: Bioassay of D-Tubocurarine

Rabbits recover immediately from the effect of curarization. They are divided into two groups each containing 4 rabbits. Minimum 8 rabbits are used.

Those rabbits which received the standard sample on the first day will be given test sample on the second day of expt. Ringer Solution 11 Ot Amount gm Nacl 6. Receptors present in Rectus muscle. Each rabbit is placed in a holder with its head protruding outside.

Pharm 1 st semester Phamacology Under the Guidance of Mr. Jyotirmoy Das Choudhury M. A linear response will be obtained. Suitable bioasszy of d-tubocurarine is 0. Saturday, 12 May Bioassay of D-Tubocurarine.

You do not have the permission to view this presentation. The potency of test sample is calculated from the standard curve. Rabbits weighing 2 kg. God Bless you man. Automatically changes to Flash or non-Flash embed.

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First group will receive standard sample and the second group will receive the sample under test. The head should be freely movable.

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Functions of Rectus muscle. Injection should be given at a rate of 0. Presentation Description No description available. Blood supply to Rectus muscle. Location of Rectus muscle. Share to Twitter Share to Facebook. Have a nice day. Bioassay of D-Tubocurarine 1. Upload from Desktop Single File Upload. Numerous small segmental contribution coming from lower inter costal arteries. D-tybocurarine to Application Have a question?

Plays an important role in breathing It helps to keep internal organ intact. Blood Supply It has several sources for arterial blood supply. Receptors The rectus abdominis muscle of frog mainly rich of Nicotinic receptor.

Functions It is an important postural muscle. I enjoy, result in I found exactly what I used to be looking for.